FAQ - Frequently Asked Questions
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Frequently Referenced FAQ
FAQ within [ HPLC ]
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(LC Column) Poor retention time reproducibility in batch ...
->Please check whether the mobile phase is degassed. (Reference:5 Degassing Methods : SHIMADZU (Shimadzu Corporation)) ->Please check whether the pum... Show Detail
- No：10681
- Date of Issue：2024/09/30 09:32
- Category： LC Columns , LC Columns
- Brached FAQ
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(LC column) Retention time shifted rapidly when the aqueo...
It is recommended to increase the concentration of organic solvents or use a column that supports 100% aqueous mobile phase. Recommended product: Shim-pack ... Show Detail
- No：10682
- Date of Issue：2024/09/30 09:32
- Category： LC Columns , LC Columns
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(LC Column) General method for washing reversed-phase column
・Flush the solution that elutes the substance adsorbed on the column. Do not precipitate out the salt inside the column. If a salt may have been used, the co... Show Detail
- No：10878
- Date of Issue：2024/09/30 09:32
- Category： LC Columns , LC Columns
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(LC Column) Different peak shapes and retention times wit...
A column with a history of analysis and a new column may not yield the same results when analyzed under the same conditions. Please check that equilibration ... Show Detail
- No：10876
- Date of Issue：2024/09/30 09:32
- Category： LC Columns , LC Columns
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(LC) High column back pressure
Matrix components in the sample may be clogged. It is recommended to pass the sample through a filter of 0.45 μm or less for HPLC analysis and 0.22 μm or les... Show Detail
- No：10690
- Date of Issue：2024/09/30 09:32
- Category： LC Columns , LC Columns
- Brached FAQ
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(LC) All peaks have bad shapes.
There is a possibility that voids are formed in the column because the filler is biased due to the sudden pressure fluctuation, or the filler is dissolved du... Show Detail
- No：10689
- Date of Issue：2024/09/30 09:33
- Category： LC Columns , LC Columns
- Brached FAQ
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(LC Column) General method for washing normal phase column
->Do not flush water. Please refer to the column instruction manual for solvent types that can pass through. ->When using a non-aqueous mobile phase i... Show Detail
- No：10688
- Date of Issue：2024/09/30 09:33
- Category： LC Columns , LC Columns
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(LC Column) Column life is too short
Contamination of the mobile phase (particulates and bacteria) Contamination of the sample (Matrices, precipitates and particulates) fragments of equipment ... Show Detail
- No：10686
- Date of Issue：2024/09/30 09:33
- Category： LC Columns , LC Columns
- Brached FAQ
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(LC Column) The back pressure of the column is higher tha...
->Make sure that the column is stored according to the instructions in the manual. ->Please check that the mobile phase containing salt such as buffer... Show Detail
- No：10685
- Date of Issue：2024/09/30 09:34
- Category： LC Columns , LC Columns
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(LC Column) Different peak shapes and retention times
->A column with a history of analysis and a new column may not obtain the same results when analyzed under the same conditions.Check that equilibration is... Show Detail
- No：10684
- Date of Issue：2024/09/30 09:34
- Category： LC Columns , LC Columns
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