1. Connect the resister tube(Example: ID0.1x4m: Parts Number: 228-32722-92) to the pump outlet instead of HPLC column.

2. Fill the reservoir with purified water to replace the mobile phase in the flow line.

3. Display [COMP] in the [PARAMETER] group, record the current setting value, and then set the value to "0.45".

4. Set the flow rate to measure (eg 1 mL/min).

5. Example; For a measuring cylinder

  a) Bring the end of the tube into contact with the cylinder wall.

  b) Determine a suitable water level inside the cylinder at which the water reaches the determined start level.

  c) Stop the stopwatch when the water is pooled 5mL.

   

    NOTE: It is also possible to fix the measurement time to 2 minutes and obtain the flow rate accuracy from the weight difference before and after.

6. When you want to correct the flow rate

  a) Display [FLOW COMP FACT](ALPHA) in the [CARIBRATION] group.

  b) To increase the flow rate, input the value obtained by adding the increment (%) to the current value. To reduce the flow rate, input the value obtained by subtracting the decrement from the current value.

    Example; the measured flow rate is 1.45% less than the set flow rate and the current flow rate correction parameter is 5.00, calculate 5.00+1.45=6.45 and input the result to increase the actual flow rate by approximately 1.45%.

7. After finishing, return the setting value of [COMP] to the original value by the same procedure as in 3.