Part Number: 225-14122-01  Standard sample (200 mL)

 

Method for Preparing the Standard Sample

1. Make up the dilution solvent.
    Dilution solvent (when making approx. 1L)
    • Pure water: 800 mL
    • Methanol: 200 mL
    • Ammonium acetate: 14.5 mg
2. Make up the stock solution.
  a) Dissolve the sample compounds indicated below in 100mL of the dilution solvent described in step 1.
    • PEG200: 15 μL
    • PEG600: 1.0 μL
    • PEG1000: 150 μL
    • PPG2000: 100 μL
    This is the stock solution.
    (It is 100 times the concentration of the standard sample. However, it does not contain raffinose.)
    PEG1000 is a solid at room temperature. To weigh it, warm it to around 60ºC to liquefy it, weigh it quickly in a disposable micro pipette and then dissolve it in the solvent.

3. Make up the standard sample for auto tuning.
  a) Dilute the stock solution created in step 2 to 1/100th concentration using the dilution solution created in step 1.
  b) Dissolve raffinose in this solution to achieve a concentration of 15 mg/L.
    This completes the procedure.
    The resulting concentrations are as indicated below.
    • PEG200: 1.5 μL/L
    • PEG600: 0.1 μL/L
    • PEG1000: 15.0 μL/L
    • PPG2000: 10.0 μL/L
    • Raffinose: 15 mg/L
    NOTE: The PEG and PPG used for the standard sample easily stick to the equipment and are difficult to remove even by washing, so the pipette, containers, etc. used for weighing these compounds should not be used for the preparation of the mobile phase or reagents.
    This will cause contamination of reagents and background noise during analysis.

 

Mass of the Standard Sample

• For the positive ionization mode
    PEG(Polyethylene Glycol): HOCH₂ (CH₂OCH₂) nCH₂OH
    Auto tuning performs mass calibration using PEG+NH₄ molecules with added ammonium ions.

   

• For the negative ionization mode
    Raffinose: C₁₈H₃₂O₁₆
    Auto tuning performs mass calibration using Raffinose 503.16 (M-H) deprotonated molecules and 1007.33 (2M-H) dimer ions.

   

Part Number: 225-14985-01  Sample for instrument calibration (200 mL)

 

Method for Preparing the Standard Sample

1. Make up the dilution solvent.
   Dilution solvent (when making approx. 1L)
    • Pure water: 800 mL
    • Methanol: 200 mL
    • Ammonium acetate: 14.5 mg
2. Make up the stock solution.
  a) Dissolve the sample compounds indicated below in 100 mL of the dilution solvent described in 1 above.
    • PEG200: 15 μL
    • PEG600: 20 μL
    • PEG1000: 150 μL
    • PPG2000: 100 μL
    This is the stock solution.
    (It is 100 times the concentration of the standard sample. However, it does not contain raffinose.)
    PEG1000 is a solid at room temperature. To weigh it, warm it to around 60 °C to liquefy it, weigh it quickly in a disposable micro pipette and then dissolve it in the solvent.

3. Make up the standard sample for auto-tuning.
  a) Dilute stock solution 2 to 1/100th of the original concentration with dilution solvent 1.
  b) Dissolve raffinose in this solution to achieve a concentration of 50 mg/L.
    This completes the procedure.
    The resulting concentrations are as indicated below.
    • PEG200: 1.5 μL/L
    • PEG600: 0.1 μL/L
    • PEG1000: 15.0 μL/L
    • PPG2000: 10.0 μL/L
    • Raffinose: 50 mg/L
    NOTE: The PEG and PPG used for the standard sample easily stick to the equipment and are difficult to remove even by washing, so the pipette, containers, etc. used for weighing these compounds should not be used for the preparation of the mobile phase or reagents.
    This will cause contamination of reagents and background noise during analysis.

 

Mass of the Standard Sample

• For the positive ionization mode
    PEG(Polyethylene Glycol): HOCH₂ (CH₂OCH₂) nCH₂OH

    Auto-tuning is performed by using the PEG+NH₄ molecules with added ammonium ions.