Baseline stability is dependent on the flowrate, detector, and mobile phase. Therefore, select a mixer that is appropriate for given system configuration and analytical conditions.
Gradient Type |
Mixer |
High-Pressure Gradient |
MR 180 μL |
Low-Pressure Gradient |
MR 300 μL LPGE
(To reduce system volume, use a MR 40 μL LPGE mixer.) |
The table below shows an example of a mixer used to reduce the gradient delay volume in a high-pressure gradient system, such as for ultra high-speed analysis.
It indicates the smallest capacity mixer usable for the given combination of detector and mobile phase.
|
Using a Mobile Phase Containing Trifluoroacetic Acid |
Using a Mobile Phase Containing Formic or Acetic Acids |
Using a Mobile Phase with Low Absorption, such as Water (Including Buffer Solutions), Organic Solvents, or Mixtures Thereof |
Using a Photodiode Array Detector |
MR 180 μL |
MR 180 μL |
MR 180 μL |
Using an Absorbance Detector |
MR 180 μL |
MR 100 μL |
MR 40 μL |
Using LCMS or LCMSMS |
- |
MR 20 μL |
MR 20 μL |
If multiple detectors are connected, use the mixer with the largest capacity. For example, if using LC/MS and PDA detectors, use the MR180 μLII. If the type of mobile phase to be used is unknown, use the largest capacity mixer, which is the MR180 μLII.