(LC Column) Different peak shapes and retention ti | FAQ - Frequently Asked Questions : SHIMADZU (Shimadzu Corporation)

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No : 10684
Date of Issue : 2024/09/30 09:34
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(LC Column) Different peak shapes and retention times

The peak shape and retention time are different when analyzed on two columns with the same P/N.

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Answer

->A column with a history of analysis and a new column may not obtain the same results when analyzed under the same conditions.Check that equilibration is sufficient for a new column.
->Consider the optimum analytical conditions that can be reproduced in multiple batch of columns.