«Main Cause»
Wavelength discrepancy exceeds 1nm.
«Countermeasure»
1. Cell is not installed properly
- Install cell properly
2. Error in wavelength calibration
- Run [WAVE CALIB.] (VP function) to calibrate wavelength, then run [WAVE CHECK] to check wavelength accuracy.
3. Sample in cell is highly absorptive of UV and visible light, or large air bubbles in sample greatly restrict amount of light transmitted through cell at emission line wavelengths (656 and 254nm) of deuterium lamp or mercury lamp.
- Flush the inside of the cell with a mobile phase solvent that does not absorb light in the vicinity of above wavelength.
- Purge cell interior with air or nitrogen.
If [CHECK NO GOOD] appears again when [WAVE CHECK] is run after the above action has been taken, turn the power OFF and contact a
Shimadzu sales or service representative.