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  • No : 8363
  • Date of Issue : 2022/02/16 16:24
  • Last Updated : 2022/02/25 16:11
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(MCE) The peak detection position fluctuates

(MCE) The peak detection position fluctuates for the same microchip.
There is a difference in peak detection times between multiple microchips.
Category : 

Answer

• The temperature of the separation buffer was not sufficiently at room temperature.
→Allow 30 minutes or more to elapse the separation buffer at room temperature before preparing and setting.
 
• The correct separation buffer is not used.
→Check the reagent kit used.
 
• The microchip is clogged.
→Remove the microchips and rinse them with water as per "Inspecting and Washing Microchip Reservoirs". If the obstruction is not removed, replace with new microchips.
  Click here for "(MCE) Inspecting and Washing Microchip Reservoirs (manually)".
 
• Separation buffer and dye are not mixed evenly.
→Agitate the separation buffer solution thoroughly.
 
• A deteriorated microchip is used.
→Contamination within the microchip channels can lead to slower peak detection times.
  Perform the procedure described in "Check Analysis Performance" in instruction manual and then check the condition of the microchip. If the microchip fails in the inspection, perform the procedure described in "Chip Cleaning" or replace the microchip with a new one.
  Click here for "(MCE) Chip Cleaning".

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