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  • No : 8359
  • Date of Issue : 2022/02/16 16:24
  • Last Updated : 2022/02/25 16:13
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(MCE) Peaks do not separate

(MCE) Peaks do not separate
Category : 

Answer

• The correct separation buffer is not used.
→Check the reagent kit.
 
• The separation buffer or marker has deteriorated.
→Verify that they have been stored at the specified temperature, and that they have not exceeded their "use by" date.
 
• The microchips have deteriorated.
→Perform the procedure described in "Check Analysis Performance" in instruction manual and then check the condition of the microchip. If the microchip fails in the inspection, perform the procedure described in "Chip Cleaning" or replace the microchip with a new one.
  Click here for "(MCE) Chip Cleaning.
 
• The sample concentration or salt concentration in the sample is too high.
→Dilute the sample.
  Desalt the sample.
 
• An air bubble has formed in the syringe.
→Examine the inside of the syringe according to "Plunger Replacement". If an air bubble has formed, remove the syringe, remove the air bubble, and then reattach the syringe.
  If the symptom is not remedied, replace the syringe.
  Click here for "(MCE) Replacement and Inspection of Plunger".
 
• The prescribed sample quantity is not added to the reservoir.
  The sample amount was less than the specified amount. Moreover, there was a layer of air inside the syringe.
→Perform [Probe Rinse].
  Click here for "(MCE) Chip Wash and Probe Rinse".
 
• The ladder does not separate in the first analysis.
  Microchip washing is insufficient.
  More than two weeks have elapsed since the last analysis was performed.
→Before analysis, automatically rinse the microchips.
  Perform [Probe Rinse].
  Click here for "(MCE) Chip Wash and Probe Rinse".

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