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(MCE) Inspecting and Washing Microchip Reservoirs (manually)
(MCE) Inspecting and Washing Microchip Reservoirs (manually)
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Answer
« Purpose »
To manually clean the microchip channels and surface and confirm that the channels are unobstructed.
« Guidelines for Procedure »
• The "Remove all chips and immediately wash them" message is displayed.
The microchips were not washed because analysis did not end properly. The microchip channels and reservoirs can become blocked due to dried separation buffer.
The microchips were not washed because analysis did not end properly. The microchip channels and reservoirs can become blocked due to dried separation buffer.
• The "Chip # has an error" message is displayed (# indicates the chip position).
Click [OK] in the dialog box. An "Error occurred. Chip # is now unavailable" message will be displayed when the analysis schedule is complete.
All microchips used in analysis are washed automatically (including microchips for which an error occurred).
However, the channels and reservoirs of microchips for which an error occurred can become blocked.
Click [OK] in the dialog box. An "Error occurred. Chip # is now unavailable" message will be displayed when the analysis schedule is complete.
All microchips used in analysis are washed automatically (including microchips for which an error occurred).
However, the channels and reservoirs of microchips for which an error occurred can become blocked.
• If no message has been displayed, but adhered substances are present on or in the vicinity of the microchip reservoir.
« Requirements »
• Milli-Q ultrapure water
• Micropipette (20μL to 200μL) and pipette tips (required quantity)
• Wipes (Recommended: BEMCOT LINTFREE PS-2)
• Micropipette (20μL to 200μL) and pipette tips (required quantity)
• Wipes (Recommended: BEMCOT LINTFREE PS-2)
« Procedure »
CAUTION:
• Do NOT scrub the reservoir when removing the moisture from the surface of the microchip.
Lint from the wipe could clog the microchip channels.
• Do NOT touch the surfaces of the microchip with your bare hands.
• Do NOT scratch the microchip electrodes during handling.
If salt or other material has hardened on the electrodes, do NOT scrub the electrodes. Apply water to dissolve the salt and then gently wipe it away.
• Do NOT clean the microchip with an ultrasonic cleaner.
This will damage the microchip.
Lint from the wipe could clog the microchip channels.
• Do NOT touch the surfaces of the microchip with your bare hands.
• Do NOT scratch the microchip electrodes during handling.
If salt or other material has hardened on the electrodes, do NOT scrub the electrodes. Apply water to dissolve the salt and then gently wipe it away.
• Do NOT clean the microchip with an ultrasonic cleaner.
This will damage the microchip.
1. Perform the procedure in "Moving All Axes to Home Position".
Click here for "(MCE) Moving All Axes to Home Position".
2. Open the chip cover, and remove the microchip from the instrument.
3. Remove the microchip from the chip frame, and place it on a flat surface.
3. Remove the microchip from the chip frame, and place it on a flat surface.
4. If precipitate is evident around the reservoir, use a micropipette to drop about 20μL of Milli-Q ultrapure water onto the precipitate.
5. Wait for 5 minutes.
6. Verify that the precipitate has dissolved, and then remove the water with a wipe.
7. Fill the standard accessory disposable syringe with 1mL of Milli-Q ultrapure water.
Assemble the filter, syringe adapter, and seal tip as shown in the figure below. Apply pressure to force water through one of the reservoirs.
5. Wait for 5 minutes.
6. Verify that the precipitate has dissolved, and then remove the water with a wipe.
7. Fill the standard accessory disposable syringe with 1mL of Milli-Q ultrapure water.
Assemble the filter, syringe adapter, and seal tip as shown in the figure below. Apply pressure to force water through one of the reservoirs.
8. Apply water through one of the reservoirs and verify that water pours out through the other three reservoirs.
If water does not pour out, return to step 4, drop Milli-Q ultrapure water onto the precipitate, and extend the waiting period to 30 minutes. If the chip still remains clogged, stop using it for analysis, and replace it with a new microchip.
If water does not pour out, return to step 4, drop Milli-Q ultrapure water onto the precipitate, and extend the waiting period to 30 minutes. If the chip still remains clogged, stop using it for analysis, and replace it with a new microchip.
CAUTION: Be sure the microchip soak time is no more than 30 minutes.
There is a danger that soaking for longer periods can damage the channel coating, and lead to degraded performance.
There is a danger that soaking for longer periods can damage the channel coating, and lead to degraded performance.
9. Remove any water in the microchip reservoirs using the micropipette.
10. If the microchip surface is still dirty, wipe it gently with a wipe soaked in Milli-Q ultrapure water.
11. Place the microchip in the chip frame.
10. If the microchip surface is still dirty, wipe it gently with a wipe soaked in Milli-Q ultrapure water.
11. Place the microchip in the chip frame.
NOTE: Place the microchip in the chip frame so that the electrode side on the top of the microchip (surface with reservoirs) is located toward the back side (area without plastic) of the chip frame top (surface with the Shimadzu logo).
12. Close the chip frame, securing the microchip.
13. Turn the microchip so that the front surface faces away from you.
14. Place the microchip back in its original position in the instrument.
CAUTION: If analysis is not performed after that, perform [All Chip Washing] to prevent water residue in the microchip channels.
CAUTION: If analysis is not performed after that, perform [All Chip Washing] to prevent water residue in the microchip channels.
Click here for "(MCE) Chip Wash and Probe Rinse".
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