FAQ - Frequently Asked Questions

If adequate purging cannot be achieved with auto-purging or a suction tube does not contain liquid, for example, use the following procedure to manually purge with a syringe.
1. Insert the suction tube for the flow channel to be purged into the reservoir bottle containing the mobile phase and place the bottle on the reservoir tray.
2. Pull the solvent delivery pump drain tube out from the autosampler drain port and insert a syringe, with a needle attached, into the end of the drain tube.

 

3. Open the right panel on the solvent delivery pump and open the drain valve (A) by turning it 90 degrees counterclockwise.

 

4. Suction mobile phase into the syringe. In general, suction about 10 mL.
5. To manually purge other mobile phase ports as well, switch to a different mobile phase port and repeat the same process to suction mobile phase with the syringe.
6. When finished manually purging all flow channels, close drain valve (A) by turning it clockwise all the way until it stops.

 

7. Close the right panel on the solvent delivery pump.
8. Remove the syringe.  Perform each auto-purging step for at least three minutes.
9. Continue auto-purging each manually purged mobile phase port until all bubbles disappear from the flow channel.

Manual purging does not purge the flow channels downstream from the solvent delivery pump. To purge the flow channels downstream from the solvent delivery pump, perform auto-purging for at least three minutes on each channel, until bubbles disappear from the flow channel.