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(LCMS) Correcting Retention Time Differences between Detectors
(LCMS) Correcting Retention Time Differences between Detectors
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In systems with multiple detectors, peak retention time data can differ for each detector. When data is analyzed, the results can be corrected for those differences.
The following describes the correction procedure for a configuration that results in peaks successively appearing from LC, PDA, and MS detectors, in that order.
The following describes the correction procedure for a configuration that results in peaks successively appearing from LC, PDA, and MS detectors, in that order.
1. Open a data file obtained by data analysis.
2. Right-click on [Chromatogram View] in the [MS Data Analysis] window and then click [MS Data View Parameters] on the menu that is displayed. In the [MS Data View Parameters] sub-window, click the [Delay Time] tab.
2. Right-click on [Chromatogram View] in the [MS Data Analysis] window and then click [MS Data View Parameters] on the menu that is displayed. In the [MS Data View Parameters] sub-window, click the [Delay Time] tab.
3. For the [Mode] setting, select the [Time] checkbox.
4. Because peaks elute from the UV detector first (indicated as detector A in the window), set correction to zero seconds.
5. Compare the elution times for the UV and PDA detectors and for the UV and MS detectors and set the correction values accordingly. In this example, correction was set to 2 and 5 seconds.
6. Then the chromatogram is displayed with peak positions corrected based on the above settings.
7. Retention times can also be corrected by adjusting the tube volume between respective detectors. In that case, change the [Mode] setting to [Volume].
8. In the example above, set the tube volume between the UV and PDA detectors and between the UV and MS detectors. Then the peak positions are corrected based on the pump flowrate specified in the method and the tube volumes specified above.
4. Because peaks elute from the UV detector first (indicated as detector A in the window), set correction to zero seconds.
5. Compare the elution times for the UV and PDA detectors and for the UV and MS detectors and set the correction values accordingly. In this example, correction was set to 2 and 5 seconds.
6. Then the chromatogram is displayed with peak positions corrected based on the above settings.
7. Retention times can also be corrected by adjusting the tube volume between respective detectors. In that case, change the [Mode] setting to [Volume].
8. In the example above, set the tube volume between the UV and PDA detectors and between the UV and MS detectors. Then the peak positions are corrected based on the pump flowrate specified in the method and the tube volumes specified above.
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