FAQ - Frequently Asked Questions
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FAQ within [ HPLC ]
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Error Code:[0x529A] FATAL SUB HEATER
«Main Cause» The sub-heater cannot be controlled. «Countermeasure» Turn off the instrument and contact our sales office / distributor or our designated ... Show Detail
- No：11660
- Date of Issue：2025/05/27 15:25
- Last Updated：2025/05/28 10:00
- Category： Autosampler
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(LC) Consumables (Needle(SIL))
Appearance Show Detail
- No：11564
- Date of Issue：2025/01/28 10:54
- Last Updated：2025/01/29 14:01
- Category： Autosampler , LC Consumables
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(LC Column) Poor reproducibility of retention time betwee...
->The composition may have changed due to volatilization of the mobile phase or sample. Reprepare and check again. ->Ensure that the column is aged su... Show Detail
- No：10683
- Date of Issue：2024/09/30 09:34
- Category： LC Columns , LC Columns
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(LC Column) Different peak shapes and retention times
->A column with a history of analysis and a new column may not obtain the same results when analyzed under the same conditions.Check that equilibration is... Show Detail
- No：10684
- Date of Issue：2024/09/30 09:34
- Category： LC Columns , LC Columns
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(LC Column) The back pressure of the column is higher tha...
->Make sure that the column is stored according to the instructions in the manual. ->Please check that the mobile phase containing salt such as buffer... Show Detail
- No：10685
- Date of Issue：2024/09/30 09:34
- Category： LC Columns , LC Columns
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(LC Column) Column life is too short
Contamination of the mobile phase (particulates and bacteria) Contamination of the sample (Matrices, precipitates and particulates) fragments of equipment ... Show Detail
- No：10686
- Date of Issue：2024/09/30 09:33
- Category： LC Columns , LC Columns
- Brached FAQ
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(LC Column) General method for washing normal phase column
->Do not flush water. Please refer to the column instruction manual for solvent types that can pass through. ->When using a non-aqueous mobile phase i... Show Detail
- No：10688
- Date of Issue：2024/09/30 09:33
- Category： LC Columns , LC Columns
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(LC) All peaks have bad shapes.
There is a possibility that voids are formed in the column because the filler is biased due to the sudden pressure fluctuation, or the filler is dissolved du... Show Detail
- No：10689
- Date of Issue：2024/09/30 09:33
- Category： LC Columns , LC Columns
- Brached FAQ
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(LC) High column back pressure
Matrix components in the sample may be clogged. It is recommended to pass the sample through a filter of 0.45 μm or less for HPLC analysis and 0.22 μm or les... Show Detail
- No：10690
- Date of Issue：2024/09/30 09:32
- Category： LC Columns , LC Columns
- Brached FAQ
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(LC Column) Different peak shapes and retention times wit...
A column with a history of analysis and a new column may not yield the same results when analyzed under the same conditions. Please check that equilibration ... Show Detail
- No：10876
- Date of Issue：2024/09/30 09:32
- Category： LC Columns , LC Columns
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