Frequently Asked Questions

No peaks are output

Check the column using known analytical conditions. If the column has deteriorated, replace it. Confirm whether the solvent delivery pump is functioning pr... Show Detail

The peak shape is abnormal

Check the column using known analytical conditions. If the column has deteriorated, replace it. Connect the tubing correctly. Backflush the high pressure... Show Detail

Peak retention times vary

Pump 2-propanol to rinse the check valve. However, if a buffer solution is being used, rinse the check valve with purified water first. Then rinse it by pump... Show Detail

The pressure change is large when hig...

Disassemble and clean the high pressure valve. If disassembled cleaning is not effective, then replace the rotor and stator. Contact a Shimadzu sales or se... Show Detail

Light level is low

Confirm whether the baseline changes significantly when the cell outlet pipe is blocked. Alternatively, visually check for any bubbles flowing in the flow ce... Show Detail

Cell Temperature Control Failure

Make sure the connector is connected securely. Specify an appropriate cell temperature-control temperature. The cell temperature-control setting range vari... Show Detail

[Ready] status never appears

Close column oven door. Specify an appropriate column temperature-control temperature. The possible temperature-control range varies depending on the room ... Show Detail

The rinse solution level for the seal...

Inspect (replace) the diaphragm. Tighten the connection. If the leak persists after tightening the connection, then replace the connection. Show Detail

Pressure increases too high

Ultrasonically clean or replace the line filter. Use the specified pipe. Inspect the column pressure and replace the column if clogged. Rinse the flow ... Show Detail

Pressure does not increase

Close the drain valve. Tighten the connection. If the leak persists after tightening the connection, then replace the connection. Show Detail