->The composition may have changed due to volatilization of the mobile phase or sample. Reprepare and check again. -

->A column with a history of analysis and a new column may not obtain the same results when analyzed under the same c

->Make sure that the column is stored according to the instructions in the manual. ->Please check that the mobile

Contamination of the mobile phase (particulates and bacteria) Contamination of the sample (Matrices, precipitates and p

->Do not flush water. Please refer to the column instruction manual for solvent types that can pass through. ->Wh

There is a possibility that voids are formed in the column because the filler is biased due to the sudden pressure fluct

Matrix components in the sample may be clogged. It is recommended to pass the sample through a filter of 0.45 μm or less

A column with a history of analysis and a new column may not yield the same results when analyzed under the same conditi

・Flush the solution that elutes the substance adsorbed on the column. Do not precipitate out the salt inside the column.

It is recommended to increase the concentration of organic solvents or use a column that supports 100% aqueous mobile ph

FAQ - Frequently Asked Questions

Font Size
S
M
L

Products Search > Columns, Reagents and Consumables > LC Columns

Keyword Search

Frequently Referenced FAQ

Latest FAQ

FAQ within [ LC Columns ]

1-10 of Total 12

≪ 1 / 2page ≫

(LC Column) Poor reproducibility of retention time betwee...

->The composition may have changed due to volatilization of the mobile phase or sample. Reprepare and check again. ->Ensure that the column is aged su... Show Detail
- No：10683
- Date of Issue：2024/09/30 09:34
(LC Column) Different peak shapes and retention times

->A column with a history of analysis and a new column may not obtain the same results when analyzed under the same conditions.Check that equilibration is... Show Detail
- No：10684
- Date of Issue：2024/09/30 09:34
(LC Column) The back pressure of the column is higher tha...

->Make sure that the column is stored according to the instructions in the manual. ->Please check that the mobile phase containing salt such as buffer... Show Detail
- No：10685
- Date of Issue：2024/09/30 09:34
(LC Column) Column life is too short

Contamination of the mobile phase (particulates and bacteria) Contamination of the sample (Matrices, precipitates and particulates) fragments of equipment ... Show Detail
- No：10686
- Date of Issue：2024/09/30 09:33
- Brached FAQ
(LC Column) General method for washing normal phase column

->Do not flush water. Please refer to the column instruction manual for solvent types that can pass through. ->When using a non-aqueous mobile phase i... Show Detail
- No：10688
- Date of Issue：2024/09/30 09:33
(LC) All peaks have bad shapes.

There is a possibility that voids are formed in the column because the filler is biased due to the sudden pressure fluctuation, or the filler is dissolved du... Show Detail
- No：10689
- Date of Issue：2024/09/30 09:33
- Brached FAQ
(LC) High column back pressure

Matrix components in the sample may be clogged. It is recommended to pass the sample through a filter of 0.45 μm or less for HPLC analysis and 0.22 μm or les... Show Detail
- No：10690
- Date of Issue：2024/09/30 09:32
- Brached FAQ
(LC Column) Different peak shapes and retention times wit...

A column with a history of analysis and a new column may not yield the same results when analyzed under the same conditions. Please check that equilibration ... Show Detail
- No：10876
- Date of Issue：2024/09/30 09:32
(LC Column) General method for washing reversed-phase column

・Flush the solution that elutes the substance adsorbed on the column. Do not precipitate out the salt inside the column. If a salt may have been used, the co... Show Detail
- No：10878
- Date of Issue：2024/09/30 09:32
(LC column) Retention time shifted rapidly when the aqueo...

It is recommended to increase the concentration of organic solvents or use a column that supports 100% aqueous mobile phase. Recommended product: Shim-pack ... Show Detail
- No：10682
- Date of Issue：2024/09/30 09:32

1-10 of Total 12

To Top of Page

This site uses the FAQ system "PKSHA FAQ", which is a product of PKSHA Communication Inc.